3M™ Allergen Protein ELISA Kit

3M

** Please contact us for pricing & details. Online orders will not be accepted.

 

3M™ Allergen Protein ELISA Kit

 

intended for the detection of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

 

The 3M Allergen Protein ELISA Kits utilize a sandwich ELISA. The target protein present in the sample react with the specific antibodies, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, antibodies conjugated with horseradish peroxidase (HRP) are added. This enzyme-labeled antibody forms complexes with the previously bound target protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of target protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of the target protein in the test sample. The quantity of protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

  • Designed to detect both processed and unprocessed target allergen proteins.
  • Test can accommodate environmental swab, CIP rinse water and food samples.
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time.

 

Allergen Proteins

Catalog Number

Assay Time

Limit of Detection

Almond

E96ALM

50 min

1.9 ng/mL (ppb)

Bovine Casein

E96CAS

50 min

1.7 ng/mL (ppb)

Bovine Total Milk

E96MLK

50 min

5.8 ng/mL (ppb)

Cashew

E96CHW

50 min

2.6 ng/mL (ppb)

Coconut

E96COC

50 min

9.9 ng/mL (ppb)

Egg White

E96EGG

50 min

2.1 ng/mL (ppb)

Fish

E96FSH

50 min

2.7 ng/mL (ppb)

Hazelnut

E96HZL

50 min

1.9 ng/mL (ppb)

Macadamia

E96MAC

50 min

1.1 ng/mL (ppb)

Mustard

E96MUS

50 min

3.1 ng/mL (ppb)

Peanut

E96PNT

50 min

6.7 ng/mL (ppb)

Pecan

E96PEC

50 min

3.7 ng/mL (ppb)

Pistachio

E96PST

50 min

3.5 ng/mL (ppb)

Sesame

E96SES

50 min

3.7 ng/mL (ppb)

Soy

E96SOY

50 min

11.0 ng/mL (ppb)

Walnut

E96WAL

50 min

6.9 ng/mL (ppb)

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