Idex MicroTee & Cross for Capillary Tubing

MicroTee and Cross for Capillary Tubing

  • Direct connect 1/16”, 1/32”, 360 μm OD tubing, plus other capillary tubing
  • Low swept volume

Use Upchurch ScientificR MicroTees and MicroCrosses to join capillary tubing. All of these products are made entirely of PEEK™ and have 0.006” (0.150 mm) thru-holes, with resulting swept volumes ranging from 29 to 81 nL.

Please Note: Use only the ferrules supplied with each connector - they are not interchangeable. Replacement ferrules and female nuts are available.

Application NOTE:

Several researchers use our PEEK MicroTee to introduce ionizing voltage to their fluid stream just prior to a Mass Spectrometer 1. MicroTees are well suited for this application due to advantageous internal geometry and PEEK polymer’s electrical resistance. The materials required for this setup are as follows: one gold or platinum conducting wire, one P-775 or P-875 MicroTee, one MicroTight Tubing Sleeve for the conducting wire (as needed to accomodate wire diameter), and at least two more MicroTight Tubing Sleeves to connect your capillary tubing. Connect the wire to the center port of the MicroTee. First, thread your wire through the appropriate tubing sleeve, if necessary, with the wire extending beyond both ends of the sleeve. Slip the female nut included with the MicroTee over the wire or sleeved wire, followed by the ferrule — ensuring the wire (and its sleeve) extends well past the end of the ferrule tip. Align the tip of the wire with the thru-hole of the MicroTee and gently insert the wire until it bottoms out. Now finger tighten the female nut into place. Attach your flow path tubing to the MicroTee’s two other available ports, following the instructions provided with the MicroTee. Begin fluid flow through the tee and apply voltage to the conducting wire lead. This setup typically provides effective electrospray ionization in applications having a flow rate of 100 μL/min or greater.

1 One such paper describing pioneering electrospray work: Protein Identification at the Low Femtomole Level from Silver-Stained Gels Using a New Fritless Electrospray Interface for Liquid Chromatography- Microspray and Nanospray Mass Spectrometry. Christine L. Gatlin, Gerd R. Kleemann, Lara G. Hays, Andrew J. Link, John R. Yates III (1998) Analytical Biochemistry 263, 93-101.

 

Serial Number

Description

P-775

MicroTee Assembly .025in

P-777

MicroCross Assembly .025in

P-885

MicroTee PEEK 1/32" w/Fittings

P-887

MicroCross PEEK 1/32" w/Fittings

P-888

MicroTee PEEK 360µm w/Fittings

P-889

MicroCross PEEK 360µm w/Fittings

P-890

MicroTee PEEK - 1/16" w/Fittings

P-891

MicroCross PEEK - 1/16" w/Fittings

P-777-01

MicroCross Body .025in

P-887-01

MicroTee Body PEEK 1/32"

P-888-01

MicroTee Body PEEK 360µm

P-890-01

MicroTee Body PEEK - 1/16"

P-885-01

MicroTee PEEK 1/32"

 

 

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