Idex Unpacked Semi-Prep Guard Column

Unpacked Semi-Prep Guard Column

  • 10 mm ID column protection
  • Convenient cartridge system
  • Easy to pack

 

The internal volume of this Upchurch Scientific® semi-prep guard column is just 780 μL, which only requires approximately 1.50 g of packing material — ideally the same material used in your semi-prep column. The C-1000 Holder will hold to high pressures, and is specially treated to prevent galling.* Use standard 10-32 coned fittings (not included) to connect your 1/16” OD tubing.

 

* Galling is a form of “cold welding.” When two fittings manufactured from the same metal are wrench-tightened too tightly, they can “weld” together, making it virtually impossible to separate the two components.

 

Application NOTE:

Low Pressure Drop

  • 60:40 Acetonitrile : Water
  • 50 mL/min
  • Kromasil 10 μm C18
  • Backpressure: 100 psi

 

Why use guard columns?

  • Columns damaged by strong or irreversible adsorption include changes in retention time and selectivity, offset baseline (bleed) and peak shape deterioration. Columns damaged by particulates (from the sample matrix, mobile phase, etc.) commonly exhibit peak splitting and increased back pressure. Guard columns protect your column in two ways. First, they act as filters trapping particles in their frits or packed bed, or both. Second, when the guard column is packed with the same material as in the analytical column, it removes compounds that irreversibly or strongly bind to the packing material. By either approach, guard columns can increase your column life considerably.

 

 

 

Serial Number

Description

C-1000

Semi-Prep Guard Column Holder

 

 

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