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Eluent degassing with PN7520 Solvent Degasser is a critical and important step in FFF, SEC and HPLC, as oxygen and other dissolved gases affect the analysis for various reasons. Because of different pressure ratios and different temperatures along the flow path, dissolved gases can be released. This will influence the flow rate stability and detector signal noise of the analysis. Especially for analysis methods using Light Scattering, UV and RI, vacuum degassing method allows an operation with lowest possible detection limits due to more smooth and stable baseline conditions.