Idex RheBuild® Kits

RheBuild® Kits

RheBuild Kits are available for all RheodyneR brand products. Included in each individualized RheBuild Kit are all parts, tools, and instructions to maintain precision performance of your particular product. RheBuild Kits eliminate individual part ordering.

Application NOTE:

How to Avoid Pressure Transients - 

Air in the sample loop can cause an instantaneous system pressure drop that eventually returns to a normal level. Air causes the pressure to drop when the injector moves from the LOAD to the INJECT position. When large sample loops (≥100 μL) are partially loaded, air present in the needle port tube is pushed into the sample loop (see Figure 1). Air can also enter the sample loop from siphoning which occurs when the vent line is higher than the injection port. In either case, upon injection, the system pressure collapses the air bubble, causing pressure to drop momentarily. A pressure drop in the system caused by air results in changes in retention time, artifact peaks, and affects column performance. Avoid pressure drops by removing the air in the needle port tube. Do this by flushing about 1 mL of mobile phase with a luer syringe with needle port cleaner. Keep the needle port tube filled with mobile phase by occasional flushing. Adjust the vent line(s) so the outlet is at the same horizontal level as the needle port. For additional injection troubleshooting, refer to the Rheodyne Troubleshooting Guide for HPLC Injection Problems. You may download the Guide from the IDEX Health & Science web site: www.idex-hs.com under Support.